Imaging individual mRNA molecules using multiple singly labeled probes.
Abstract
We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons.
MeSH terms
Animals
Caenorhabditis elegans
Drosophila melanogaster
In Situ Hybridization, Fluorescence
Mammals
Molecular Probes
RNA, Messenger
Authors
Recommend literature
1. Visualization of single RNA transcripts in situ.
2. RNA imaging. Spatially resolved, highly multiplexed RNA profiling in single cells.
3. Single-cell systems biology by super-resolution imaging and combinatorial labeling.
4. Single mRNA Molecule Detection in Drosophila.
5. Single-cell in situ RNA profiling by sequential hybridization.
Similar data
1. Single-cell RNA-seq reveals intrinsic and extrinsic regulatory heterogeneity in yeast responding to stress
2. Molecular, Spatial and Functional Single-Cell Profiling of the Hypothalamic Preoptic Region
3. Genome-wide co-localization of RNA-DNA interactions and fusion RNA pairs (RNAseq)
4. Genome-wide co-localization of RNA-DNA interactions and fusion RNA pairs (iMARGI)
5. Prokaryotic Single-Cell RNA Sequencing by In Situ Combinatorial Indexing