Imaging individual mRNA molecules using multiple singly labeled probes.
IF: 47.990
Cited by: 1,620


We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons.

MeSH terms

Caenorhabditis elegans
Drosophila melanogaster
In Situ Hybridization, Fluorescence
Molecular Probes
RNA, Messenger


Raj, Arjun
van den Bogaard, Patrick
Rifkin, Scott A
van Oudenaarden, Alexander
Tyagi, Sanjay

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