STOmics technology:BGI Stereomics Stereo-Seq

Data type:Spatial transcriptomics

Sample scope:Monoisolate

Summary:T cells and B cells mediate antigen-specific inflammation. Tracing T cell and B cell clonal activity through heritable receptor sequences could provide significant insights on immune surveillance and regulation, while current available tools could not provide clonal information at single cell resolution with spatial distribution simultaneously. To break through this, we developed an ultra-sensitive T/B cell receptor sequencing strategy based on stereo-seq. Thus, we yielded a spatially resolved immune repertoire atlas at single cell resolution and observed heterogenic LAs with varied clonal activity in perturbed tissues. Specifically, 50um-2000um micro aggregates formed in a kidney cancer for T and B cell clonal expansion. Intratumoral tertiary lymphoid structure (TLS) primed significantly higher proportion of mutated B/plasma clones over peritumoral TLS in lung cancer. In inflammatory bowel disease, immigrant B/plasma cells matured in the plasma cell aggregates within the lamina propria, instead of the submucosal TLS, to mediate locoregional inflammation. Collectively, our study provides an effective approach to profile spatial immune repertoire at single cell resolution with paired TCR/BCR chains, allowing us to decipher T/B cell clonal activities crossing perturbed tissues.

Contributor(s):Yu Feng.

Submitter:刘怡(Yi Liu),BGI

Release date:2024-12-29

Updated:2024-12-29