STOmics technology:BGI Stereomics Stereo-Seq

Organism(s):--

Data type:Spatial transcriptomics

Sample scope:Monoisolate

Summary:The gradual transition of pluripotent callus from root to shoot identity drives shoot regeneration. However, cell heterogeneity and their spatial organization during this cell-fate transition remain unclear, hindering our mechanistic understanding and utilization of plant regeneration. Here, we presented the first comprehensive time-series transcriptomic landscape of the shoot regeneration process at tissue, single-cell, and spatial resolution. During the early stage, we identified a crucial transition cell type, developing SAM (dSAM), which showed distinct dual root-shoot meristem characteristics. Further spatial transcriptomics analysis revealed that dSAM was derived from the QC-like cell (QCL) and eventually differentiated into a shoot system. Moreover, we found that PDL and QCL cells acted as dSAM surrounding barriers and created an isolated cytokinin-rich environment, facilitating shoot cell-fate establishment and development. Finally, the activation of epidermal development regulators ATML1 and MYB31 facilitated dSAM transition. These findings provide new perspectives to understand cell diversity and spatial organization during shoot regeneration.

Contributor(s):Li Jiming, Qin Chao, Pan Yajie, Xu Mengyuan, Xia Keke et al.

Submitter:秦超(Qin Chao),BGI-Shenzhen

Release date:2025-06-01