1Public Datasets of STOmicsSource: STOmics DB (ID: STT0000124 )

Browse and obtain STOmics public datasets for further exploration needs.

2Single Cell Multi-Omic reveal the impact of salt stress on NHCC root tip developmentSource: STOmics DB (ID: STT0000066 )

Single Cell Multi-Omic reveal the impact of salt stress on NHCC root tip development

3The Spatial Transcriptomics of upland rice rootSource: STOmics DB (ID: STT0000026 )

Upland rice and irrigated rice have different root patterning adapted to rainfall or irrigated conditions, respectively. Due to the increasing scarcity of water for irrigation, it is necessary to study the mechanism of root patterning in upland rice. Here we investigated the fine difference in root between these two ecotypes and found that upland rice have less crown root (CR), longer meristem and more cortex in root tip than irrigated rice. We used a high-resolution spatial transcriptomics technology, Stereo-seq, to dissect the transcriptional differences in the CR-producing coleoptile and root tips.

4Spatial transcriptomic study of alveolar echinococcosisSource: STOmics DB (ID: STT0000072 )

Alveolar echinococcosis (AE) is characterized by increased infiltration of various immune cells around the lesion. To investigate the spatial transcriptional dynamics in AE lesions, we conducted Stereo-seq to generate the spatiotemporal transcriptomic atlas of mouse livers collected at multiple timepoints after Echinococcus multilocularis infection.

5Spatial transcriptome sequencing of human early tooth developmentSource: STOmics DB (ID: STT0000140 )

Spatial transcriptome seguencing of human early tooth development at late bud, cap and early bell stages.

6Spatiotemporal transcriptomic atlas of atherosclerosis revealed Smooth muscle cells induce tertiary lymphoid organs in plaqueSource: STOmics DB (ID: STT0000052 )

Tertiary lymphoid organs (TLOs) develop within nonlymphoid tissues in response to persistent inflammation, However, there is limited knowledge regarding their presence in atherosclerosis diseases and the mechanisms underlying their development. Here, we used single-cell RNA sequencing and Spatial Enhanced Resolution Omics-sequencing (Stereo-seq) to obtain a comprehensive plaque atlas comprising 30 distinct transcriptome-defined cell types. Notably, we have observed the presence of TLOs within plaques, and their formation is closely associated with the expression of the lymphorganogenic chemokine, CXCL12, produced by fibroblast-like smooth muscle cells (SMCs) in a LTβR-dependent manner. In plaque TLOs (PTLOs), B cells were abundant and capable of differentiating into IgA- and IgG- plasma cells. BCR repertoire analysis unveiled clonal diversification, selection, expansion in PTLO, and the presence of identical B cell clonotypes in other regions of the plaques. Interestingly, we also identified the co-occurrence of unique B cell clonotypes in plaques and perivascular adipose tissue (PVAT) by using lineage analysis, indicating cell exchange between these compartments. PTLO-derived IgG antibodies promote the differentiation macrophages into foam cells, suggestive of pro-atherogenic effector activity. Additionally, the presence of PTLOs is linked with cardiovascular events. Thus, the PTLOs induced by fibroblast-like SMCs play key roles in the development and stability of atherosclerosis plaques, by conferring B cell maturation and IgG antibody production.

7A Spatiotemporal Dynamic Immune Landscape of the COVID-19 Hamster Lung [Spatiotemporal]Source: STOmics DB (ID: STT0000006 )

Although SARS‐CoV‐2‐mediated inflammation has attracted global health concerns since 2019, its pulmonary immunopathology is not fully understood. Here we generated a comprehensive cellular and molecular landscape of healthy and COVID-19 hamster lungs at different timepoints after infection, using single-cell RNA sequencing and spatial transcriptomic sequencing to map the entire progression of COVID-19. We found SARS-CoV-2 could infect naïve T cells and induced cell death to decrease T cell number at the early stage of COVID-19. Besides, we observed the activation and depletion of tissue resident myeloid cells after infection, the accumulation of Isg12+Cst7+ neutrophils and Il10+Spp1+ M2-like macrophages to clean up virus and resolve inflammation. Finally, we identified Trem2+AM and Fbp1+AM subsets during the resolution stage of COVID-19. Our study provided spatiotemporally-resolved insights into the lung cells transcriptome, identified distinct tissue regions of viral infection, lung injury, repair and remodeling.

8Spatiotemporal transcriptomics elucidates the pathogenesis of fulminant viral myocarditisSource: STOmics DB (ID: STT0000127 )

Fulminant myocarditis (FM), a severe myocardial inflammatory disease, frequently leads to sudden death in young individuals. This study presents a comprehensive transcriptome analysis of coxsackievirus B3 (CVB3)-induced myocarditis in A/J mice, both pre- and post-treatment, utilizing single-cell and spatial transcriptomics at seven distinct time points. Our research uncovers the pivotal role of mesothelial cells in the pathogenesis of myocarditis, serving as the initial targets for CVB3 that subsequently activate macrophages, fostering an inflammatory environment. This activation cascade leads to the enrichment of macrophages and T cells in the affected myocardial regions, where they contribute to tissue damage through the development of pro-inflammatory 'Inflammatory_Mac' from peripheral monocytes. Moreover, our findings highlight the detrimental impact of Cd8+ effector T cells, which, upon releasing cytotoxic molecules, especially IFN-γ, modulate the expression of Spi1, driving cardiomyocyte death and further exacerbating the disease's progression. Targeting both IFN-γ and Spi1 yield positive treatment effects on FM mice. As an important immunomodulatory drug, Intravenous immunoglobulin (IVIG) treatment significantly reduced the death rate of FM mice, inhibits the proliferation of virus and suppresses the hyperinflammatory state of inflamed heart. The expression of IFN-γ and Spi1 also decreased. These highlighted the therapeutic potential of IVIG and IFN-γ/Spi1 axis targeted therapy. The comprehensive spatiotemporal omics analysis, conducted pre- and post-FM treatment, has offered profound insights, significantly advancing our understanding and facilitating the strategic management of FM.

9An Organ-wide Spatiotemporal Transcriptomic and Cellular Atlas of the Regenerating Zebrafish HeartSource: STOmics DB (ID: STT0000071 )

Adult zebrafish robustly regenerate injured hearts through a complex orchestration of cells and molecules. However, the comprehensive process remains incompletely understood. Here, we utilized single-cell RNA-sequencing (scRNA-seq) and Stereo-seq approaches to generate a spatially-resolved cell dataset of regenerating zebrafish heart. We captured organ-scale cellular dynamics across eight time points, with a particular focus on the initiative stages. We reconstructed a 4D "virtual regenerating heart" atlas, encompassing 3 spatial dimensions and time, comprising a total of 569,896 cells/spots derived from 36 scRNA-seq libraries and 224 Stereo-seq slices.

10Multi-omics project research on germ-free mice.Source: STOmics DB (ID: STT0000129 )

The gut microbiota influences host immunity and metabolism, and changes in its composition and function have been implicated in several non-communicable diseases. Here, comparing germ-free (GF) and specific pathogen-free (SPF) mice using spatial transcriptomics, single-cell RNA sequencing, and targeted bile acid metabolomics across multiple organs, we systematically assessed how the gut microbiota's absence affected organ morphology, immune homeostasis, bile acid, and lipid metabolism. Through integrated analysis, we detect marked aberration in B, myeloid, and T/NK cells, altered mucosal zonation and nutrient uptake, and significant shifts in bile acid profiles in feces, liver, and circulation, with the alternate synthesis pathway predominant in GF mice, and pronounced changes in bile acid enterohepatic circulation.Particularly, autophagy-driven lipid droplet breakdown in ileum epithelium and the liver's zinc finger and BTB domain containing protein (ZBTB20)- Lipoprotein lipase (LPL) axis are key to plasma lipid homeostasis in GF mice. Our results unveil the complexity of microbiota-host interactions in the crosstalk between commensal gut bacteria and the host.