Insulin-like growth factor I reduces coronary atherosclerosis in pigs with familial hypercholesterolemia(Dataset ID: STDS0000280)

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PMID: 36602878 (IF:8.0 Q1)
Summary
Data
Sample
Catalog
Dataset information
Contributors
Accessions
How to cite
Dataset information
Summary:
To investigate effects of IGF-1 on human-like coronary atherosclerosis we used high fat diet-fed Rapacz pigs with familial hypercholesterolemia (FH pigs). We used spatial transcriptomics (ST) analysis to identify global transcriptome changes in advanced plaque compartments and to obtain mechanistic insights into IGF-1 effects. IGF-1-injected FH pigs had 1.8-fold increase in total circulating IGF-1 levels compared to control. IGF-1 decreased relative coronary atheroma (data obtained by serial intravascular ultrasound) and lesion cross-sectional area (post-mortem histology). IGF-1 increased fibrous cap thickness, and reduced necrotic core size, macrophage content, and cell apoptosis, changes consistent with promotion of a stable plaque phenotype. ST analysis revealed that IGF-1 suppressed FOS/FOSB factors and gene expression of MMP9 and CXCL14 suggesting possible involvement of these molecules in IGF-1’s effect on atherosclerosis.
Overall design:
FH pigs were fed with high-fat diet and received daily injections of IGF-1 or saline (control) for 6 months. A total 4 cryosections of right coronary artery (RCA) were obtained from female FH pigs (N=2, IGF-1-treated pigs, N=2, saline-treated pigs, control) and used for spatial transcriptomics (ST). Section imaging, RNA extraction, cDNA library preparation and sequencing were performed in according to the ST’s manufacturer (Visium/10X genomics) recommendations.
Technology:
ST
Platform:
NextSeq 1000
Species:
Sus scrofa
Tissues:
Right coronary artery
Development stage:
20 months
Sex:
Female
Submission date: 2022-12-06Update date: 2023-12-04
DOI: To be continue
Contributors
Sukhanov, Sergiy; Yoshida, Tadashi; Higashi, Yusuke; Delafontaine, Patrice
Contact: contrib1
Accessions
GEO Series Accessions: GSE220218
How to cite
  • Cite database of STOmicsDB:
    [1] Xu, Zhicheng et al. "STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization." Nucleic acids research vol. 52,D1 (2024): D1053-D1061. doi: 10.1093/nar/gkad933'
  • Cite visualization dataset:
    [2] Sukhanov, Sergiy. Insulin-like growth factor I reduces coronary atherosclerosis in pigs with familial hypercholesterolemia[DS/OL]. STOmicsDB, 2022[2022-12-06]. https://db.cngb.org/stomics/datasets/STDS0000280/. doi: xxxxxx
    #Format: {contributors}. {title}[DS/OL]. STOmicsDB, {the year of submission data}[{submission data}]. {dataset link}. doi: {doi ID}
  • Cite original data article:
    No results.