MCNSTA: Mouse Cochlear Nucleus Spatiotemporal Transcriptomic Atlas(Dataset ID: STDS0000243)
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Summary:
"
The molecular and cellular mechanisms underlying the function of the cochlear nucleus (CN) remain to be fully elucidated. Using single-nucleus RNA sequencing and single-cell spatial transcriptome analyses, we identified transcriptome-defined cell types, as well as their spatial distribution and marker genes. These data also allowed us to acquire a new definition of CN subregions. By comparing transcriptomic profiles between normal mice and mutant mice with congenital hearing loss due to hair cell malfunction, we further identified glutamatergic Spp1+-bushy cell as a primary cell type exhibiting hearing loss-induced alteration in gene expression. Among highly affected genes in the bushy cell, we found that deletion of a developmentally regulated osteopontin-encoding gene Spp1 affected CN processing of auditory signals. Together, our study provides the most comprehensive molecular and cellular atlas of CN to date and identifies critical hearing loss-related cell types and specific genes that may serve as potential therapeutic targets. "Overall design:
to be continuedTechnology:
Stereo-Seq
Platform:
DNBSEQ-T1
Species:
Mus musculus
Tissues:
Brain
Organ parts:
Cochlear nucleus
Cell types:
to be continued
Sex:
Male | FemaleSubmission date: 2024-08-30Update date: 2024-09-05
Contributors
Liu Huihui; liaoshangfeng
Contact: liuhent9h@163.com, liaoshangfeng@genomics.cn
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No results. Reference way
Citation: to be continued