Macaque Hypothalamus Atlas for Obesity and Diabetes(Dataset ID: STDS0000238)
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Summary:
Although the hypothalamus plays a crucial role in the progression of metabolic diseases including obesity and diabetes, the underlying molecular remodeling remains unclear. Here we systematically compared the transcriptomic features of structured hypothalamus at single-cell resolution in obese and diabetic macaques. We found that the infundibular (INF) and the paraventricular nucleus (PVN) were the most susceptible regions to metabolic disruption with the PVN being more sensitive to diabetes. In the INF, obesity displayed reduced synaptic plasticity and energy sensing capability. By contrast, in diabetes there was interactive molecular reprogramming associated with an impaired tanycyte barrier, activated microglia and stimulation of neuronal inflammatory response. Results from Stereo-seq further demonstrate a unique spatial tropism of microglia towards parenchyma relative to the third ventricle. Our data provide an extensive reference resource of distinct molecular changes between obesity and diabetes in the primate hypothalamus, which may facilitate more precise and effective therapies for metabolic disorders.Overall design:
We conducted sampling of the arcuate nucleus (INF), ventromedial nucleus (VMH), dorsomedial nucleus (DMH), paraventricular nucleus (PVN), and lateral hypothalamic area (LHA) of the hypothalamus in three healthy macaques, three obese macaques fed a high-fat diet, and two spontaneous diabetic macaques for scRNA-seq.Additionally, we performed spatial stereo-seq sequencing on hypothalamic tissue samples from one healthy macaque and three spontaneous diabetic macaques.Technology:
Stereo-Seq, RNA-seq
Platform:
DNBseq
Species:
Macaca fascicularis
Tissues:
Brain
Organ parts:
Hypothalamus
Cell types:
oligodendrocyte, microglia, vascular cells, astrocyte, ependymal cells, tanycytes, inhibitory neuron, excitatory neuron, oligodendrocyte progenitor cel
Submission date: 2024-03-22Update date: 2024-03-22
Sample number: 12Section number: 9
Contributors
Ying Lei
Contact: leiying1@genomics.cn
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No results.