Reconstruction of the Mouse Olfactory Glomerular Map using Single-Cell Technologies
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Dataset information
Summary:
The goal of scRNA-seq is to explore whether olfactory sensory neurons (OSNs) that display a specific olfactory receptor (OR) express a distinct transcriptional program from OSNs that display a different OR in a large and unbias scale. Olfactory epithelial single cell suspensions were made using 3 male and 3 female mice. Single-cell sequencing libraries were prepared using Chromium Single Cell 3’ Reagent Kit V3 (10X Genomics) according to the manufacturer’s guidelines. Libraries were sequenced using 150 cycles of paired end reads on Illumina Hiseq4000 and Novaseq6000 instruments (Novogene). The sequencing reads were processed using the DolphinNext Single Cell-10X Genomics pipeline (https://dolphinnext.umassmed.edu/index.phOverall design:
Olfactory epithelial single cell suspensions made from 3 male and 3 female adult mice. Single-cell sequencing libraries were prepared using Chromium Single Cell 3’ Reagent Kit V3 (10X Genomics) according to the manufacturer’s guidelines. Libraries were sequenced using 150 cycles of paired end reads on Illumina Hiseq4000 and Novaseq6000 instruments (Novogene)Technology:
MERFISH
Platform:
Illumina NovaSeq 6000 (Mus musculus)
Species:
Mus musculus(mm10)
Tissues:
Epithelium
Organ parts:
Main olfactory epithelium
Sex:
maleCitation:
Wang, I-Hao et al. “Spatial transcriptomic reconstruction of the mouse olfactory glomerular map suggests principles of odor processing.” Nature neuroscience vol. 25,4 (2022): 484-492. doi:10.1038/s41593-022-01030-8Submission date: 2021-03-16Update date: 2022-05-02
Contributors
Wang, I-Hao; Jiang, Hao-Ching; Park, Sung J; Liberman Greer, Paul
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