Reconstruction of the Mouse Olfactory Glomerular Map using Single-Cell Technologies(Dataset ID: STDS0000161)

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PMID: 35314823
Summary
Data
Sample
Catalog
Dataset information
Contributors
Accessions
How to cite
Dataset information
Summary:
The goal of scRNA-seq is to explore whether olfactory sensory neurons (OSNs) that display a specific olfactory receptor (OR) express a distinct transcriptional program from OSNs that display a different OR in a large and unbias scale. Olfactory epithelial single cell suspensions were made using 3 male and 3 female mice. Single-cell sequencing libraries were prepared using Chromium Single Cell 3’ Reagent Kit V3 (10X Genomics) according to the manufacturer’s guidelines. Libraries were sequenced using 150 cycles of paired end reads on Illumina Hiseq4000 and Novaseq6000 instruments (Novogene). The sequencing reads were processed using the DolphinNext Single Cell-10X Genomics pipeline (https://dolphinnext.umassmed.edu/index.ph
Overall design:
Olfactory epithelial single cell suspensions made from 3 male and 3 female adult mice. Single-cell sequencing libraries were prepared using Chromium Single Cell 3’ Reagent Kit V3 (10X Genomics) according to the manufacturer’s guidelines. Libraries were sequenced using 150 cycles of paired end reads on Illumina Hiseq4000 and Novaseq6000 instruments (Novogene)
Technology:
MERFISH
Platform:
Illumina NovaSeq 6000 (Mus musculus)
Species:
Mus musculus(mm10)
Tissues:
Epithelium
Organ parts:
Main olfactory epithelium
Sex:
male
Submission date: 2021-03-16Update date: 2022-05-02
DOI: To be continue
Contributors
Wang, I-Hao,Jiang, Hao-Ching,Park, Sung J,Liberman Greer, Paul
Accessions
Data url: link
How to cite
  • Cite database of STOmicsDB:
    [1] Xu, Zhicheng et al. "STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization." Nucleic acids research vol. 52,D1 (2024): D1053-D1061. doi: 10.1093/nar/gkad933'
  • Cite visualization dataset:
    [2] Wang, I-Hao,Jiang, Hao-Ching,Park, Sung J,Liberman Greer, Paul. Reconstruction of the Mouse Olfactory Glomerular Map using Single-Cell Technologies[DS/OL]. STOmicsDB, 2021[2021-03-16]. https://db.cngb.org/stomics/datasets/STDS0000161/. doi: xxxxxx
    #Format: {contributors}. {title}[DS/OL]. STOmicsDB, {the year of submission data}[{submission data}]. {dataset link}. doi: {doi ID}
  • Cite original data article:
    Citation: Wang, I-Hao et al. “Spatial transcriptomic reconstruction of the mouse olfactory glomerular map suggests principles of odor processing.” Nature neuroscience vol. 25,4 (2022): 484-492. doi:10.1038/s41593-022-01030-8