LCM-Seq analysis of microscopically defined human embryo HSC microenvironment
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Dataset information
Summary
This dataset contains RNA-Seq data for cells within spatially defined subdomains of the human embryo dorsal aorta, subdissected from tissue sections using laser capture microdissection. This dataset explores dorsal-ventral polarisation across the dorsal aorta, the site of hematopoietic stem cell emergence.
This dataset contains RNA-Seq data for cells within spatially defined subdomains of the human embryo dorsal aorta, subdissected from tissue sections using laser capture microdissection. This dataset explores dorsal-ventral polarisation across the dorsal aorta, the site of hematopoietic stem cell emergence.
Overall design
In total 30 samples (subdomains) from 6 human embryos are analysed. This includes 6 subdomains (V_Inner, D_Inner, V_Mid, D_Mid, V_Outer, D_Outer) from 3 CS16 embryos and 4 subdomains ([V], [VL], [DL], [D]) from 3 CS17 embryos.
In total 30 samples (subdomains) from 6 human embryos are analysed. This includes 6 subdomains (V_Inner, D_Inner, V_Mid, D_Mid, V_Outer, D_Outer) from 3 CS16 embryos and 4 subdomains ([V], [VL], [DL], [D]) from 3 CS17 embryos.
Technology
LCM-seq
LCM-seq
Platform
GPL21103
GPL21103
Species
Homo sapiens
Tissues
Dorsal aorta
Dorsal aorta
Development stage
developmental stage:CS17, developmental stage:CS16
developmental stage:CS17, developmental stage:CS16
Citation
Crosse, Edie I et al. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development.Cell Stem Cell. 2020 Nov 5;27(5):822-839.e8. doi: 10.1016/j.stem.2020.08.004. Epub 2020 Sep 17.
Crosse, Edie I et al. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development.Cell Stem Cell. 2020 Nov 5;27(5):822-839.e8. doi: 10.1016/j.stem.2020.08.004. Epub 2020 Sep 17.
Submission date: 2020-06-05Update date: 2020-12-01
Accessions
GEO Series Accessions:
GSE151875