Comprehensive Atlas of the Mouse Urinary Bladder
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Dataset information
Summary:
We report the application of single-cell based sequencing for high throughput profiling of the mouse urinary bladder. By utilizing multiple dissociation techniques and library preparation techniques we generated an atlas comprising 43,119 cells including major cell types absent from previous reports. We found that previous single-cell profiling of the mouse bladder lacked a major cell type in the detrusor smooth muscle and incorrectly annotated other cell types such as mesothelial cells. Using the atlas, we elucidated aspects of bladder biology including urothelial differentiation, the identity of interstitial cells of Cajal, detrusor smooth muscle control and immune distributions. Finally, we combine the single-cell based sequencing with spatial transcriptomics and imaging mass cytometry to add spatial context to transcriptomic profiling.Overall design:
Single cell and nucleus sequencing of the mouse urinary bladder paired with spatial transcriptomics and bulk RNA sequencing.Technology:
10x Visium
Platform:
GPL21103
Species:
Mus musculus(mm10)
Tissues:
Bladder
Organ parts:
Whole Bladder Nuclear, Whole Mouse Bladder, Whole Bladder, Whole Mouse Bladder, Dissociated Mouse Bladder, Detrusor layer of bladder
Citation:
Lemtiri-Chlieh, Fouad et al. “The hyperpolarization-activated, cyclic nucleotide-gated channel resides on myocytes in mouse bladders and contributes to adrenergic-induced detrusor relaxation.” American journal of physiology. Regulatory, integrative and comparative physiology vol. 323,1 (2022): R110-R122. doi:10.1152/ajpregu.00277.2021Submission date: 2021-07-14Update date: 2021-12-23
Sample number: 4
Contributors
Dylan Baker; Paul Robson
Contact: baker@uchc.edu
Accessions
GEO Series Accessions:
GSE180128