Spatiotemporal role of the lncRNA Evx1as in embryonic patterning
Summary:We performed spatial transcriptome analysis on cryosections of gastrulating embryos from E6.5 to E7.5 by low-input RNA sequencing (Geo-seq). Careful spatial transcriptomic analysis revealed that the lncRNA Evx1as plays a transient spatiotemporal role in regulating embryonic patterning during gastrulation, yet is dispensable for overall development and survival. Altogether, these results suggest that many lncRNAs are generally dispensable for normal development and survival, but may be selectively required in a particular context in vivo.
Overall design:All RNA-seq(s) were designed to reveal the spatiotemporal role of lncRNA Evx1as in embryonic patterning.
embryonic stem cells, 4x polyA KI in embryonic stem cells, Evx1as+/- proximal posterier, Evx1as+/- distal posterier, Evx1as-/- proximal posterier, Evx1as-/- distal posterier, Evx1as+/- Primtive streak layer, Evx1as+/- mesoderm layer, Evx1as+/- anterior epiblast cells, Evx1as-/- Primtive streak layer, Evx1as-/- mesoderm layer, Evx1as-/- anterior epiblast cells
Embryonic 7 days
Citation:Han, Xue et al. “Mouse knockout models reveal largely dispensable but context-dependent functions of lncRNAs during development.” Journal of molecular cell biology vol. 10,2 (2018): 175-178. doi:10.1093/jmcb/mjy003
Submission date: 2017-09-26Update date: 2021-07-25
Luo S; Peng G; Cui G; Lu J; Jing N; Shen X
AccessionsGEO Series Accessions: GSE104292