Spatial transcriptome analysis of gastric cancer which GAN-KP transplanted C57BL/6J mouse
Summary:We have now developed an organoid-based model of gastric cancer from GAstric Neoplasia (GAN) mice, which express Wnt1 and the enzymes COX2 and microsomal prostaglandin E synthase 1 in the stomach. Both p53 knockout (GAN-p53KO) organoids and KRASG12V-expressing GAN-p53KO (GAN-KP) organoids were generated by genetic manipulation of GAN mouseâ€“derived tumor (GAN-WT) organoids. To uncover the molecular mechanism underlying the intratumoral heterogeneity of GAN-KP tumors, we performed spatial transcriptomics analysis with the 10Ã— Genomics Visium platform, which allows characterization of the spatial topography of gene expression.
Overall design:GAN-KP cells were transplanted into the stomach wall of mice and gene expression was analyzed two weeks later.
Citation:Yamasaki, Juntaro et al. “MEK inhibition suppresses metastatic progression of KRAS-mutated gastric cancer.” Cancer science vol. 113,3 (2022): 916-925. doi:10.1111/cas.15244
Submission date: 2021-10-20Update date: 2021-10-24
Juntaro Yamasaki; Yuki Hirata; Hideyuki Saya; Osamu Nagano
AccessionsGEO Series Accessions: GSE186290