Spatial transcriptome analysis of gastric cancer which GAN-KP transplanted C57BL/6J mouse(Dataset ID: STDS0000093)

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485
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164
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Spots: 2,429
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Genes: 31,054
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PMID: 34931404
Summary
Visualization
Data
Analysis results
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Dataset information
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Dataset information
Summary:
We have now developed an organoid-based model of gastric cancer from GAstric Neoplasia (GAN) mice, which express Wnt1 and the enzymes COX2 and microsomal prostaglandin E synthase 1 in the stomach. Both p53 knockout (GAN-p53KO) organoids and KRASG12V-expressing GAN-p53KO (GAN-KP) organoids were generated by genetic manipulation of GAN mouse–derived tumor (GAN-WT) organoids. To uncover the molecular mechanism underlying the intratumoral heterogeneity of GAN-KP tumors, we performed spatial transcriptomics analysis with the 10× Genomics Visium platform, which allows characterization of the spatial topography of gene expression.
Overall design:
GAN-KP cells were transplanted into the stomach wall of mice and gene expression was analyzed two weeks later.
Technology:
10x Visium
Platform:
GPL17021
Species:
Mus musculus(mm10)
Tissues:
Stomach
Organ parts:
GAN-KP tumor
Submission date: 2021-10-20Update date: 2021-10-24
DOI: To be continue
Contributors
Juntaro Yamasaki,Yuki Hirata,Hideyuki Saya,Osamu Nagano
Accessions
GEO Series Accessions: GSE186290
How to cite
  • Cite database of STOmicsDB:
    [1] Xu, Zhicheng et al. "STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization." Nucleic acids research vol. 52,D1 (2024): D1053-D1061. doi: 10.1093/nar/gkad933'
  • Cite visualization dataset:
    [2] Juntaro Yamasaki,Yuki Hirata,Hideyuki Saya,Osamu Nagano. Spatial transcriptome analysis of gastric cancer which GAN-KP transplanted C57BL/6J mouse[DS/OL]. STOmicsDB, 2021[2021-10-20]. https://db.cngb.org/stomics/datasets/STDS0000093/. doi: xxxxxx
    #Format: {contributors}. {title}[DS/OL]. STOmicsDB, {the year of submission data}[{submission data}]. {dataset link}. doi: {doi ID}
  • Cite original data article:
    Citation: Yamasaki, Juntaro et al. “MEK inhibition suppresses metastatic progression of KRAS-mutated gastric cancer.” Cancer science vol. 113,3 (2022): 916-925. doi:10.1111/cas.15244