Spatial transcriptome analysis of gastric cancer which GAN-KP transplanted C57BL/6J mouse
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Summary:
We have now developed an organoid-based model of gastric cancer from GAstric Neoplasia (GAN) mice, which express Wnt1 and the enzymes COX2 and microsomal prostaglandin E synthase 1 in the stomach. Both p53 knockout (GAN-p53KO) organoids and KRASG12V-expressing GAN-p53KO (GAN-KP) organoids were generated by genetic manipulation of GAN mouse–derived tumor (GAN-WT) organoids. To uncover the molecular mechanism underlying the intratumoral heterogeneity of GAN-KP tumors, we performed spatial transcriptomics analysis with the 10× Genomics Visium platform, which allows characterization of the spatial topography of gene expression.Overall design:
GAN-KP cells were transplanted into the stomach wall of mice and gene expression was analyzed two weeks later.Technology:
10x Visium
Platform:
GPL17021
Species:
Mus musculus(mm10)
Tissues:
Stomach
Organ parts:
GAN-KP tumor
Citation:
Yamasaki, Juntaro et al. “MEK inhibition suppresses metastatic progression of KRAS-mutated gastric cancer.” Cancer science vol. 113,3 (2022): 916-925. doi:10.1111/cas.15244Submission date: 2021-10-20Update date: 2021-10-24
Contributors
Juntaro Yamasaki; Yuki Hirata; Hideyuki Saya; Osamu Nagano
Accessions
GEO Series Accessions:
GSE186290