Spatial transcriptomics of human brain metastases
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Dataset information
Summary:
Spatial transcriptomics of six surgically-resected human brain metastasis samplesOverall design:
After surgical resection, parts of six human brain metastases were embedded in OCT and frozen in a dry ice/2-methylbutane bath. 10 脦录M sections were placed on Visium gene expression slides which were stored at -80C until use. Slides were methanol fixed and stained with H&E and imaged at 10x on a Biotek Lionheart microscope. Tissues were permeabilized and libraries generated according to the manufacturer's protocols. Images for all samples are available in all_spatial_images.tar.gz.Technology:
10x Visium
Platform:
GPL24676
Species:
Homo sapiens(hg38)
Tissues:
Brain
Citation:
Hudson, William H, and Lisa J Sudmeier. “Localization of T cell clonotypes using the Visium spatial transcriptomics platform.” STAR protocols vol. 3,2 101391. 10 Jun. 2022, doi:10.1016/j.xpro.2022.101391Submission date: 2021-07-06Update date: 2021-08-06
Contributors
William H Hudson
Accessions
GEO Series Accessions:
GSE179572