Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns(Dataset ID: STDS0000079)
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Summary:
To advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used spatial | transcriptomics to generate high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region- and tissue-specific genes. | We have found extensive sex-specific gene expression differences in the germline and sperm and discovered genes that are specifically expressed in the male reproductive tract. These include a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, enabling transcriptome-wide comparisons of gene expression patterns.Overall design:
Spatial transcriptomics by RNA-tomography (TomoSeq) on 20um sectionsTechnology:
Tomo-seq
Platform:
GPL13657
Species:
Caenorhabditis elegans(WS249)
Submission date: 2018-05-21Update date: 2018-11-05
Contributors
Annabel Ebbing; Abel Vertesy; Marco Betist; Bastiaan Spanjaard; Jan P Junker; Eugene Berezikov; Alexander van Oudenaarden; Hendrik C Korswagen
Accessions
GEO Series Accessions:
GSE114723
Reference way
Citation: Ebbing, Annabel et al. “Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns.” Developmental cell vol. 47,6 (2018): 801-813.e6. doi:10.1016/j.devcel.2018.10.016