Single-cell spatial transcriptomic analysis reveals common and divergent features of developing postnatal granule cerebellar cells and medulloblastoma(Dataset ID: STDS0000013)

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Spots: 24,919
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Genes: 14,146
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PMID: 34210306
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Dataset information
Summary:
The progenitor/differentiation states of cerebellum granule cells have been investigated for many years. Deregulation of granule cells development is associated with the pathogenesis of medulloblastoma (MB).Our study reveals the cellular and spatial organization of cerebellar granule cells and provide direct evidence showing the similarity and discrepancy between normal development and tumorigenesis
Overall design:
Single cell mRNA profiles of 2 samples from Math1-GFP mice, 2 samples from Dcx-DsRed mice, 2 samples from WT mice and 3 SHH MBs from Patched+/- mice were generated by 10x Genomics plartform, without technical replication, and sequenced by NovaSeq 6000 (Illumina). Spatial transcriptomics (ST) profiles of 2 samples of P7 WT mice were generated by 10x Genomics plartform, without technical replication, and sequenced by NovaSeq 6000 (Illumina).
Technology:
Spatial Transcriptomics, scRNA
Platform:
Illumina NovaSeq 6000
Species:
Mus musculus(mm10)
Tissues:
Cerebella
Cell types:
Cerebellar granule neuron progenitors
Disease:
SHH medulloblastoma
Submission date: 2020-08-21Update date: 2021-06-14
Sample number: 10Section number: 1
Contributors
Wang J; Gao W
Contact: wj860520@163.com
Accessions
GEO Series Accessions: GSE156633
Reference way
Citation: Luo, Wenqin et al. “Single-cell spatial transcriptomic analysis reveals common and divergent features of developing postnatal granule cerebellar cells and medulloblastoma.” BMC biology vol. 19,1 135. 1 Jul. 2021, doi:10.1186/s12915-021-01071-8