1 Job name(optional)
2PCR template

Enter FASTA sequence or drag FASTA file(limit 10MB).

Primer range(optional):

3 Primer parameters

Primer direction:

Primer length(optional):

Primer product size range(optional):

Sequence internal excluded region(optional):

Primer

Introduction

The primary function of the Primer is primer design. In PCR experiments, primers are a critical component that binds to the template DNA and initiates the amplification of the DNA. Primer can automatically design primers with good specificity based on the input DNA sequence, which is convenient for users to perform PCR amplification.

The specificity of the primers can be verified using the BLAST tool.

https://db.cngb.org/blast/

Data format

1.You can enter FASTA sequence or upload FASTA file. File format:txt, fasta, fa.

2.File size limit of 10MB.

Run demo

When running the demo data, the sequencing data is:

>example1
GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG

and primer parameters are default parameters.

Result

The output result is as follows:

The file prefix is the filled sequence ID, and for, int, and rev represent the forward primer, intermediate primer, and reverse primer.