ZESTA: Zebrafish Embryogenesis Spatiotemporal Transcriptomic Atlas

ZESTA employed Stereo-seq to dissect the spatiotemporal dynamics of gene expression and regulatory networks in the developing zebrafish embryos. We profiled 91 embryo sections covering six critical time points during the first 24 hours of development, obtaining a total of 152,977 spots at a resolution of 10x10x15 µm3 (close to cellular size) with spatial coordinates. Meanwhile, we identified spatial modules and co-varying genes for specific tissue organizations. By performing the integrated analysis of the Stereo-seq and scRNA-seq data from each time point, we reconstructed the spatially resolved developmental trajectories of cell fate transitions and molecular changes during zebrafish embryogenesis.

Release at 2022-05-04

Liu, C., Li, R., Li, Y., Lin, X., Zhao, K., Liu, Q., ... & Liu, L. (2022). Spatiotemporal mapping of gene expression landscapes and developmental trajectories during zebrafish embryogenesis. Developmental Cell, 57(10), 1284-1298. DOI: 10.1016/j.devcel.2022.04.009