Project name: Medicago truncatula

Description: We used an M. truncatula 16K oligonucleotide array to profile gene expression in the leaves of transgenic alfalfa plants expressing Medicago truncatula isoflavone synthase 1 (MtIFS1). RNA purified from leaves of MtIFS1-expressing lines C22 and B20 and vector control line VC11 was used to generate Cy5-labeled cDNA. RNA purified from a second vector control line, VB2, was used to generate Cy3-labeled reference cDNA. Three independently propagated cuttings of each line were used and a total of nine hybridizations were performed. Our results indicated that MtIFS1-expression does not significantly alter global gene expression in the leaves.Keywords = MedicagoKeywords = isoflavone synthaseKeywords = alfalfaKeywords: other

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2005-07-20

Last updated: 2005-04-19