Project name: Integrative analyses of transcriptome and proteome provides new insights into energy metabolism and light utilzation in cigar tobacco during different light intensities

Description: In this study, we have used the comparative transcriptomic and proteomic approaches to decipher the changes in genes and protein abundances in cigar tobacco leaves under LL. In this study, DEGs and DEPs related to glycolysis, starch and sucrose metabolism, tyrosine metabolism, photosynthesis-antenna proteins, and photosynthesis pathways are significantly enriched. This study offers novel insights into both transcriptome and proteome levels response mechanisms under different light intensities.Overall design: The cigar tobacco H382 variety was used as test material in this study。A total of nine RNA samples were prepared from three treatments and three biological replicates (3╳3 = 9). Total RNA was extracted from the leaves samples using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) consulting manufacturer instructions. GOseq R package was used to identify significantly enriched GO terms and KOBAS software was used to test the statistical enrichment of differential expression genes in KEGG pathways. qRT-PCR was performed using SYBR Premix Ex Taq II (Takara, Japan).

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Agricultural

Last updated: 2022-08-23