Project name: HSFA1a controls plant heat stress response through its action on the 3D chromatin reorganization of enhancer-promoter interactions [HiChIP]

Description: We investigated the role of HSFA1a, a master regulator of heat stress response, in this reorganization through promotion of the formation of promoter/enhancer chromatin loops.To validate the presence of transcription factories we performed a HiChIP experiment, which is a sensitive and efficient method to analyze protein-centric chromosome conformation, using an anti-RNA polymerase II antibody.Overall design: Comparative interaction profiling analysis of HiChIP_RNAPII assay data for tomato M82 wild type after 0h and 1h of heat stress.

Data type: Other

Sample scope: Multiisolate

Relevance: Agricultural

Last updated: 2022-06-17