Project name: Glycine max cultivar:Clark

Description: We used bean pod mottle virus (BPMV) vectors to perform virus induced gene silencing (VIGS) on the soybean (Glycine max) cultivar Clark. Six BPMV-VIGS vectors we created targeting three genes individually, two combinations of two genes in the same vector, and an empty vector to serve as a control. The three individual genes were GmGLU1 (Glyma.03G128300), GmARR4 (Glyma.03G130000), and GmbHLH38 (Glyma.03G130400 and Glyma.03G130600). We used the Gibson Assembly method to create two vectors that combined GmARR4+GmGLU1 or GmbHLH+GmGLU1 in order to target two genes simultaneously. All plants were grown in iron sufficient hydroponics.

Data type: transcriptome

Sample scope: Multiisolate

Relevance: Agricultural

Last updated: 2021-11-03