Project name: Hordeum vulgare

Description: RNAseq was performed on several barley accessions that vary for their reaction to wheat stripe rust (Puccinia striiformis f. sp. tritici) to identify markers and understand the gene content at the Rps8 (Resistant to Puccinia striiformis f. sp. tritici 8) locus. Accessions were sequenced using either 100 or 150 bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.36) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Tophat (version 2013-11-10) with default parameters.

Data type: raw sequence reads

Sample scope: Multiisolate

Relevance: Agricultural

Last updated: 2021-09-08