Project name: Transcriptome changes induced by bitter melon (Momordica charantia)- intake in a high-fat diet induced obesity model

Description: Metabolic effects of lyophilized bitter melon juice (BMJ) extract (oral gavage 200mg/kg/body weight-daily for 40 days) intake were evaluated in diet-induced obese C57BL/6J male mice [fed-high fat diet (HFD), 60 kcal% fat]. Changes in gene expression in the hepatic transcriptome (microarray analysis using Affymetrix Mouse Exon 1.0 ST arrays)Expression estimates for individual genes were estimated using Affymetrix Power Tools and the RMA-sketch algorithm. Probe sets were grouped into gene clusters using Ensembl gene annotation (version GRCm38). A probe set was included in a gene cluster if all of its associated probes aligned to a region completely contained within the exonic region of the gene.Overall design: Total of 7 samples from mice randomized into two groups: a) Control-DIO group (n = 3 per group) and b) BMJ+DIO group (n = 4 per group)

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Last updated: 2021-06-01