Project name: RNA sequencing of tomato seed tissues matured in different environments

Description: During maturation seeds acquire several physiological traits to enable them to survive drying and disseminate the species. The maternal environment during maturation can influence seed quality. RNA-seq analyses of the embryo and the endosperm of seed matured ex planta under standard or stressful condition (high temperature and/or dim light) will underly how light and temperature and a combination of both have an impact on the molecular mechanisms governing seed quality at harvest.Overall design: Fruits at breaker stage (i.e 63 DAF) were collected from mother plants grown in greenhouse and transfered to growth chambers under 4 different environments: standard temperature + high light; standard temperature + dim light; high temperature + high light or high temperature + dim light. Three or four replicates of 10 fresh tomato seeds were harvested at mature stage for each maturation environment. RNA-seq data of isolated seed tissues was obtained for each maturation condition

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Agricultural

Last updated: 2020-09-28