Project name: RNA-seq data of Phytophthora infestans 88069 infected tomato leaves

Description: In both plants and animals, alternative splicing (AS) increases transcriptome and proteome diversity, and play roles in developmental and immune responses. However, the extent of host genome-wide AS changes and how these changes are modulated by adaptive pathogens during disease remain largely unknown. Here we examined AS changes in Phytophthora infestans infected Solanum lycopersicum leaves using paired-end illumina RNA-seq. Quite a number of AS events are independent of gene differential expression, indicating that AS changes may be an additional layer of plant immunity against pathogens. we also demonstrated that P. infestans regulates plant immunity by repressing the AS of positive regulator of plant immunity or activating the AS of susceptibility factor. Furthermore, we established a splicing reporter system and screened some P. infestans splicing regulatory effectors (SREs) that are involved in plant mRNA alternative splicing process. In addition, we demonstrated that the WY motif of SRE3 is required for AS activity and virulence function. Overall, our current data reveal that P. infestans delivers SREs to disrupts host AS regulation machinery and subsequently suppress host immunity.Overall design: 16 RNA samples from the mock-inoculated control leaves and leaves that had been infected with P. infestans for 1d, 2d and 3 d, using four replicates for each treatment.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Agricultural

Last updated: 2019-11-18