Project name: Abies balsamea fungal endophytes

Description: The aim of this study was to evaluate if (1) conserving the original 5’ extremity of the fungal pyrosequences, usually the partial fragment of the ribosomal small sub-unit (pSSU) immediately adjacent to the ITS1F primer ahead of the ITS1 sub-locus, impacts the detection of chimeras; (2) as the pSSU displays a lower variability, its inclusion in addition to the ITS1 sub-locus might also buffer the variation due to putative sequencing errors, and reduce the formation of the singletons and doubletons; (3) performing ecological analyses based on pSSU-ITS1 leads to similar conclusions on the community structure as analyses based on ITS1. For this study we analysed the fungal endophytic community (endomycobiota) of a single individual of Abies balsamea (L.) Mill. based on 108 samples distributed among both the aerial and root systems.

Data type: targeted loci environmental

Sample scope: Environment

Relevance: Environmental

Last updated: 2019-06-06