Project name: Mapping the differential distribution of the cohesin subunit Scc1 in Trypanosoma brucei brucei.

Description: Chromatin immunoprecipitation of genomic loci in Trypanosoma brucei where Scc1 is deposited. This was achieved by deletion of one Scc1 allele and N-terminal tagging of the second Scc1 allele with a Ty1-tag. During the ChIP experiment, the DNA was crosslinked and cells were permeabilized with digitonin. DNA was fragmented by sonication with a Covaris instrument. DNA bound to Ty1-Scc1 was pulled down by using a BB2 anti-Ty1 antibody. Cross links are reversed and the DNA was purified and prepared for Illumina sequencing.Overall design: Experiment was done in triplicates, each with an input control. As a control, an input sample was included for each ChIP experiment.

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2018-08-27