Project name: Maps of histone variant H4.V deposition in Trypanosoma brucei brucei

Description: Chromatin immunoprecipitation of genomic loci in Trypanosoma brucei where histone variant H4.V is deposited. A previously generated cell line (Siegel et al., 2009) in which both endogenous H4.V alleles are knockout out and ectopic overexpression of a Ty1-tagged version of H4.V can be induced was used. During the ChIP experiment, the DNA was digested with MNase to obtain mononucleosomes. Nucleosomes containing H4.V were pulled down by using a BB2 anti-Ty1 antibody. Cross links are reversed and mononucleosomal DNA is purified and prepared for Illumina sequencing.Overall design: Mnase ChIPs were done in duplicates, input control samples were included for each ChIP sample.

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2018-08-22