Project name: Maps of histone variant H3.V deposition in Trypanosoma brucei brucei

Description: Chromatin immunoprecipitation of genomic loci in Trypanosoma brucei where histone variant H3.V is deposited. This was achieved by deletion of one H3.V allele and N-terminal tagging of the second H3.V allele with a Ty1 tag. During the ChIP experiment, the DNA was digested with MNase to obtain mononucleosomes. Nucleosomes containing H3.V were pulled down by using a BB2 anti-Ty1 antibody. Cross links are reversed and mononucleosomal DNA is purified and prepared for Illumina sequencing.Overall design: Mnase ChIPs were done in duplicates, input control samples were included for each ChIP sample.

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2018-08-22