Project name: RNA-Sequencing identifies differentially expressed genes in adult male tilapia following low Benzo (a) Pyrene (BaP) exposure

Description: Purpose: The objective of this study was to identifiy if a low dose of BaP would alter gene expression profiles and key pathways in the liver and testis of adult male tilapiaMethods: The liver and testis tissue from the BaP-treated or control groups fish were collected and then total RNA was extracted, RNA integrity (RIN) was evaluated (RIN >7) for Illumina RNA-Seq library construction and Sequencing. Gene read mapping was performed with TopHat and differential expression analysis (DE) was conducted using exact test with R package EdgeR (p ± 1.5 were considered as significant). Pathway analysis was performed using the gene set enrichment analysis (GSEA) and subnetwork enrichment analysis (SNEA) applying the Mann-Whitney test with an alpha level of p < 0.05 to identify the biological mechanism that underlie the BaP effects in male tilapia.Results: RNA-Seq analysis using EdgeR showed a larger number of altered genes in liver than in testis as a result of BaP exposure. In liver, 1444 genes were significantly differentially expressed, while in the testis only 309 showed any changes using a p ± 1.5Conclusions: Low dose of BaP can alter genes and molecular pathways in the liver and testis in male adult tilapia, and these changes could be associated with biological endpoints disruption.Overall design: Illumina NextSeq500 Sequencing was used to generate transcriptomic profiles for BaP-exposure liver (n=4 or control, n=4 for BaP) and testis (n=3 for control, n=3 for BaP) from adult male tilapia

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Other

Last updated: 2018-07-05