Project name: Porphyromonas gingivalis ATCC 33277

Description: Immunization against Porphyromonas gingivalis for Prevention of Experimentally Induced Periodontitis in RatsAbstractObjectives: Periodontitis is an inflammatory disease causing destruction of tooth supporting structures. It is often caused by Gram-negative microorganisms such as Porphyromonas gingivalis (P. gingivalis). Common treatments of periodontitis are often non-specific and include mechanical plaque removal and surgery. This study aimed to assess the amount of bone loss and antibody titer against P. gingivalis in rats.Materials and Methods: This in vitro experimental study was conducted on 66 Surrey rats free of black pigmented pathogens, which were randomly divided into six groups of 11. Groups 1 and 2 were vaccinated with formalin-killed whole cell (FKWC) P. gingivalis with incomplete Freund's adjuvant as vaccine carrier and groups 3 and 4 were vaccinated with incomplete Freund's adjuvant and PG buffer. Groups 5 and 6 were considered as positive and negative controls, respectively. Three weeks later, they were vaccinated with a booster dose. At 28 days, groups 1, 3 and 5 were inoculated with viable P. gingivalis (ATCC 33277) four time with 48-hour intervals for induction of periodontitis. One week after booster dose administration and two weeks after oral inoculation of bacteria, serum and saliva samples were obtained for assessment of antibody titer. Ten weeks after final bacterial inoculation, the serum and saliva samples were obtained to assess antibody titer and subgingival plaque samples were obtained from the maxillary second molar site to assess the bacterial count. The rats were then sacrificed to assess bacterial count and bone loss.Results: Serum and saliva antibody titers in groups 1 and 2 were significantly different from those in other groups one week after booster dose and two and 10 weeks after oral inoculation of bacteria (P0.99) but had a significant difference with the positive control (group 5) and group 3 (PConclusion: This study showed successful immunization against P. gingivalis, which increased serum IgG and saliva IgA titers, limited the colonization of P. gingivalis in subgingival plaque, and restricted the alveolar bone loss.

Data type: other

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2018-05-11