Project name: Genome-wide identification of genes directly regulated by ChvI and a consensus sequence for ChvI binding in Sinorhizobium meliloti

Description: We have used chromatin immunoprecipitation followed by microarray analysis (chIP-chip) to identify DNA regions bound by the ChvI protein in Sinorhizobium meliloti cells. We then used quantitative PCR with chvI mutant strains to test the ChvI-dependent expression of genes downstream of the ChvI-bound DNA regions.Overall design: Chromatin immunoprecipitation followed by custom Affymetrix GeneChip analysis (chIP-chip) to identify DNA regions enriched for binding of ChvI

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: Environmental

Last updated: 2018-04-06