Project name: Expression data from tomato root tissue inoculated or not with a plant growth promoting bacteria (PGPB) strain

Description: Possitive effects of plant growth promoting bacteria (PGPB) inoculation on plant growth and development are dependent on interaction between bacterial strains and plant roots, which are usually the bacterial niche. Furthermore, phytohormones are key regulators of plant physiology. Ethylene is essential in plant growth and development and in response to drought. Plant sensibility to ethylene is involved in plant response to PGPB strain inoculation and plant growth promotion.We used microarrays to detail the global programme of gene expression underlying plant interaction with two different PGPB strains (isolated from arid soils in southern Spain) regarding to plant sentitivity to ethylene by tomato ethylene receptor 3 (SlETR3).Overall design: Ethylene-insensitive never ripe and its isogenic wild-type cv. Pearson lines were inoculated with either Bacillus megaterium or Enterobacter sp. C7 and grown until mature stage (eight weeks post-inoculation) under well-watered and drought conditions. The present study pursued to assess the bacterial effects on gene expression in tomato (Solanum lycopersicum). Root tissue samples inoculated or not with a bacterial strain were selected for RNA extraction and hybridization on Affymetrix microarrays. To that end, we selected three samples per treatment according to plant sensitivity to ethylene, watering regime and inoculated bacteria: wild type cv Pearson (WT) and never ripe (NR) plants under well-watered and drought (D) conditions non-inoculated (NO, controls), or inoculated either with Bacillus megaterium (BM), or Enterobacter C7 (C7).

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Agricultural

Last updated: 2017-10-30