Project name: Expression data from leaflets and roots of the tomato res mutant and its background genotype, wild-type (WT) (Solanum lycopersicum L. cv. Moneymaker), grown in control conditions and salt stress

Description: The res (restored cell structure by salinity) mutant exhibits a remarkable growth inhibition and morphological alterations in roots and leaves, which are suppressed when the mutant plants are exposed to salinity. The analysis of mutants like res may be very helpful in understanding how plants are able to coordinate the balance between growth and stress, a fundamental and not fully-understood question of important agronomical implicationsIn order to understand the molecular basis of phenotype recovery and tolerance induced by salt stress in res mutant, we carried out a comparative transcriptomic analysis between res and WT plants. This analysis revealed a constitutive alteration of an important number of genes involved in different pathways in res mutant, and identified genes specifically overexpressed under salt stress and responsible of both maintaining plant growth and promoting stress tolerance in res mutant.Overall design: Gene expression was analysed in roots and leaves of WT and res plants in absence of stress (control) and when the phenotypic recovery of res mutant began to be observed after salt stress (5 days of 200 mM NaCl). Leaflets (from the first fully-expanded leaf) and roots were harvested and immediately frozen in liquid N2 for subsequent RNA extraction. Three biological replicates (each one including three plants) were considered for each genotype and treatment, resulting in a total of 24 samples. Microarrays analysis was performed using the GeneChip® Tomato Gene 1.1 ST Array Strip (Affymetrix).

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Agricultural

Last updated: 2017-10-25