Project name: Casuarina glauca cultivar:Casuarina glauca Sieber ex Spreng

Description: Casuarina glauca belongs to a family of angiosperms called actinorhizal plants because they can develop nitrogen-fixing nodules in association with the soil bacteria Frankia. For de novo transcriptome, 10 symbiotic conditions were sampled: root systems from inoculated plants were harvested at the following times: 2, 4, 8, 10, 14, 21 days after inoculation with Frankia and 3 weeks old nodules were also sampled. Mycorrhized roots were collected 6 weeks after inoculation with Rhizophagus irregularis. All samples were immediately frozen in liquid nitrogen. Non symbiotic conditions ie root with or without nitrogen and leaves. Total RNA was purified from roots and nodules by ultracentrifugation (Chirgwin et al., 1979).Residual DNA was removed from RNA samples using the Turbo DNA free kit (Ambion, USA), quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Courtaboeuf, France) and qualitatively assessed using a Bioanalyser 2100 (Agilent p/n G2938A Santa Clara, CA, USA). For each sample, 2,5 μg of total RNA was pooled in a single sample, that was used to generate a normalized cDNA library (GATC Biotech) and then used for 454 Roche GS-FLX 454 Titanium sequencing.Contact: Valérie HOCHER, IRD. Valerie.hocher@ird.fr

Data type: transcriptome

Sample scope: Monoisolate

Relevance: Agricultural

Last updated: 2017-08-03