Project name: Trypanosoma brucei brucei

Description: RNA was extraced from wildtype and deletion mutants and a comparative analysis of gene expression was performed using DESeq2.Overall design: Total RNA from 45 million cells was extracted in triplicates, spiked with a synthetic RNA control. RNA was depleted for rRNA using custom-made anti-rRNA oligonucleotides that hybridize to the target RNAs and are subsequently digested with RNAseH. RNA was reverse transcribed into cDNA and libraries for Illumina sequencing were constructed. We aimed at 15 million reads for each replicate. Comparative expression analysis was performed using DeSeq2.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2017-07-06