Leaf transcriptome sequencing and assembly of several barley accessions
Source: NCBI BioProject (ID PRJNA378723)
Source: NCBI BioProject (ID PRJNA378723)
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Project name: Hordeum vulgare subsp. vulgare
Description: RNAseq was performed on several barley accessions to understand their diversity, with an emphasis on the class of plant immune receptors that contain nucleotide-binding, leucine-rich repeats (NB-LRR). The accessions were sequenced at either 100 bp or 150 bp paired end, except CI 16147 which had 150 bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.32) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Tophat (version 2013-11-10) with default parameters.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: Agricultural
Organization: The Sainsbury Laboratory
Last updated: 2017-03-10