Expression analysis of Thermococcus onnurineus NA1 KCTC10859 and an Frh-deficient mutant, Frh overexpression mutant and TON_0282 deletion mutant in a T. onnurineus NA1 strain.
Source: NCBI BioProject (ID PRJNA348351)
Source: NCBI BioProject (ID PRJNA348351)
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Project name: Thermococcus onnurineus NA1
Description: Investigation of whole genome gene expression level changes in an Frh-deficient mutant, Frh overexpression mutant and TON_0282 deletion mutant, compared to the wild-type strain.In this study, as a first step to assess the role of the frhAGB-encoding hydrogenases and TON_0282 gene, we constructed three mutants by deleting each gene in a T. onnurineus NA1.Overall design: A custom microarray was manufactured by Roche NimbleGen. Unique six 60-mer oligonucleotides for all of the predicted 1,986 open reading frames (ORFs) in the annotated genome of T. onnurineus NA1 were designed and synthesized. Examined Wildtype, Frh-deficient mutant, Frh overexpression mutant and TON_0282 deletion mutant in T. onnurineus NA1 strain.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: Other
Organization: Department of Marine Biotechnology, Korea Institute of Ocean Science and Technology
Last updated: 2016-10-13