Genome wide RNAi screen for regulators of mRNAs silenced in African trypanosome slender forms and expressed in stumpy forms
Source: NCBI BioProject (ID PRJNA322510)
Source: NCBI BioProject (ID PRJNA322510)
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Project name: Trypanosoma brucei
Description: Bloodstream forms of T brucei s427 2T1 T7 p’5neo5’-ESAG9 3’UTR (long form) bloodstream forms were grown in vitro. They contained the NeoR gene under the control of the ESAG9 gene 3'UTR. Selection was based on increased resistance to G418 through the silencing of an ESAG9 repressor, leading to increased neoR expressionOverall design: RNA library insert amplicons were analysed by ion torrent sequence analysis of the selected populations
Data type: Other
Sample scope: Multiisolate
Relevance: Medical
Organization: Centre for Immunity, Infection and Evolution
Literatures
- PMID: 28334017
Last updated: 2016-05-23