Project name: Yarrowia lipolytica

Description: The goal of this study is to find out how Yarrowia lipolytica W29’s transcriptome changes in response to different pHs in certain medium, where the production of citric acid and lipid vary significantly at different pHs. The changes in transcriptome will give us insight about the mechanism of citric acid and/or lipid production are regulated. We harvested Y. lipolytica cells from bioreactor cultures from three different pHs: 2.0, 4.0 and 6.0 in triplicates. We sequenced the mRNA by Illumina HiSeq 2500 and generated an average of 20 million reads for each sample. We did not find distinct gene expression changes for the enzymes right downstream the intersection where citrate is cleaved into acetyl-CoA. Instead, from gene ontology enrichment analysis, we observed proteins with transport activities were overrepresented in gene groups that were up-regulated higher pH conditions, where citric acid secretion is favored.Overall design: mRNA profiling of Yarrowia lipolytica W29 cells in pH 2.0, 4.0 and 6.0 bioreactors in triplicates, harvested at hour 50, sequenced by Illumina HiSeq 2500

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Industrial

Last updated: 2016-04-27