RIP-Seq analysis of Trypanosoma brucei ELAV-like protein Tb927.8.6650
Source: NCBI BioProject (ID PRJNA197395)
Source: NCBI BioProject (ID PRJNA197395)
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Project name: Trypanosoma brucei
Description: A tagged ectopic version of the ELAV-like protein Tb927.8.6650 of T. brucei was expressed in stable cell lines and pulled down. Co-purifying transcripts were analyzed by sequencing to identify RNAs associated with Tb927.8.6650.Overall design: Stable procyclic form cell lines expressing tetracycline-inducible TAP-tagged Tb927.8.6650 were created. Cells were harvested 48h after tet-induction, followed by tandem affinity purification of Tb927.8.6650, extraction of co-purified RNA, and sequencing.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: Medical
Organization: Computational and Statistical Genomics Lab, Human Genetics, McGill University
Literatures
- PMID: 23877242
Last updated: 2013-04-17