Project name: Sinorhizobium meliloti

Description: Within this work we identified and characterized SMc03169 (hhrA) as a new Sinorhizobium meliloti gene product with relevance to biological nitrogen fixation symbiosis with leguminous plants. HhrA belongs to the TetR-family of repressors and its deletion from S. meliloti genome affected considerably gene expression as well as several phenotypic traits.Overall design: For expression profiling, triplicates of the wild-type Sinorhizobium meliloti 1021 or the SMc03169 (delta hhrA) deletion mutant were grown in GMS liquid medium supplemented with 10 micromolar of luteolin for 4 hours at 30ºC and 180 rpm, followed by total RNA extraction.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Environmental

Last updated: 2013-02-23