Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1 (Illumina smRNA-Seq]
Source: NCBI BioProject (ID PRJNA190016)
Source: NCBI BioProject (ID PRJNA190016)
0 0
Project name: Drosophila melanogaster
Description: Salivary glands or larval ovaries were isolated from transgenic flies expressing RNAi targeting Nautilus (control) or linker histone H1 using a Tub-Gal4 driver.Overall design: ~200 larvae were used to isolate salivary glands or ovaries, independently. Total RNA was isolated using Trizol reagent following manufacturer's guidelines. Then 5 µg of total RNA was separated on a polyacrylamide gel, and 18-29 nt small RNAs were isolated for cloning.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: ModelOrganism
Organization: Dr. Art Skoultchi, Cell Biology, Albert Einstein College of Medicine
Literatures
- PMID: 23559249
Last updated: 2013-02-19