Activation of seed-specific genes in leaves and roots of the desiccation-tolerant plant, Xerophyta humilis
Source: NCBI BioProject (ID PRJNA150967)
Source: NCBI BioProject (ID PRJNA150967)
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Project name: Xerophyta humilis
Description: Measurement of changes in the mRNA transcript abundance of 1709 cDNAs in desiccated (5% RWC) and hydrated (100% RWC) Xerophyta humilis leaves and roots, and in mature seeds.Overall design: 3105 cDNA clones (corresponding to 1709 unique cDNAs) were randomly selected from X. humilis cDNA libraries (Leaf Dehydration (LD), Leaf Rehydration (LR), Root Dehydration (RD) and Root Rehydration (RR)), amplified by PCR, and printed on each glass slide multiple times (ranging from 4 to 12X), such that each printed block contained a random unbiased mixture of cDNAs from the 4 different cDNA libraries. Total RNA extracted from each X. humilis leaf, root and seed sample was labelled with Cy3 and hybridized to the printed cDNA arrays. Data was recorded for three biological replicates for each of the samples being investigated.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: Other
Organization: Evo-Devo, Lab 426, Molecular and Cell Biology, University of Cape Town
Release date: 2012-03-30
Last updated: 2012-01-09