Project name: Glycine max

Description: Transgenic soybean plants expressing AtBBX32 are taller, and have more nodes, flowers, pods, and seed. The effects of expression of AtBBX32 are most evident in altering expression of a subset of genes in the early morning hours. In particular, AtBBX32 alters expression of the soybean clock genes TOC1 and LHY1This experiment compares the response of AtBBX32 transgenic plants with a control plant under field grown conditions at five time points.Overall design: Four lines, five timepoints and three biological replicates for a total of 60 samples. Of the four lines, three were AtBBX32 transgenic lines (of which two were high yielding [Event1 and Event2], one was not [Event4]), and a non-transgenic control event [A3244].Sample times: 3AM, 6AM, 9AM, Noon, and 3PM (ZT21, ZT0, ZT3, ZT6 and ZT9) (Sunrise was at 6:08 AM and Sunset was at 8:08 PM)Development Stage and Tissue: Trifoliate leaf at R5 stage (80 days post-emergent)Each of the three bioreps was a pool of three plants.The field was located in central Illinois

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Agricultural

Release date: 2012-03-01

Last updated: 2011-07-20