Project name: Homo sapiens

Description: DNA methylation profiling of human B cell populations representing a developmental series before and after immune activation. The B cells are derived from inflamed tonsils. The B cells were already activated in vivo in patients, therefore there was no need to stimulate the B cells after isolation.Gene expression profiling was also performed from the same samples.Overall design: Four B cell subsets: Naïve, germinal center B cells (GC), memory B cells, and plasma cells (PC) were purified by FACS ex vivo from tonsils of 8 human subjects. Methylated CpG island Recovery assay (MIRA) was utilized to enrich for methylated DNA fragments from each sample for microarray analysis.*** This Series reports DNA methylation profiling data. ***

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2010-10-25