Project name: Arabidopsis thaliana

Description: Formaldehyde is a toxic volatile organic compound and its mechanism of toxicity to plant has not yet been revealed. This experiment was designed to identify formaldehyde-responsible genes under exposure to low or high concentration of airborne formaldehyde for a short period of time. 7 weeks old Arabidopsis thaliana transformant (ecotype: Columbia) plants were exposed to gaseous formaldehyde at 1-2 ppm (low), 14-16 ppm (high), or less than 0.04 ppm (air control) at 24oC under light-condition for 150 minutes inside a chamber for formaldehyde exposure. Total RNA was isolated from rosette leaves of exposed plants and was applied to microarray analysis. We investigated into formaldehyde dose response on gene expression of Arabidopsis and tried to understand the toxic mechanisms of formaldehyde using an Affymetrix Arabidopsis genome array ATH-1.Overall design: In this study, we used Arabidopsis thaliana Columbia double transgenic plants that expressed 3-hexulose-6-phosphate synthase gene (rmpA) and 6-phospho-3-hexuloisomerase gene (rmpB) from Mycobacterium gastri MB19 in chloroplasts (Chen et al. 2010). The transgenic plants (Arabidposis AB plants) can assimilate and tolerant to formaldehyde. 7 weeks old Arabidopsis thaliana (Col) AB plants were exposed to gaseous formaldehyde at 1-2 ppm (low), 14-16 ppm (high), or less than 0.04 ppm (air control) at 24oC under light condition for 150 minutes inside of exposure chamber. When exposed under each condition, 3 plants were placed inside the exposure chamber, and these plants were treated as replications. In addition to treatment samples, non-treatment plants that were placed outside the chamber at 24oC under light-condition for 150 minutes until exposure were also prepared. Total RNA was isolated from rosette leaves of plants. In total, 12 samples were used in this experiment.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Last updated: 2010-03-12