3h pathway stimulation / inhibition experiment in epiblast stem cells
Source: NCBI BioProject (ID PRJNA123651)
Source: NCBI BioProject (ID PRJNA123651)
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Project name: Mus musculus
Description: This experiment records the transcriptional responses of EpiSCs (line E3) to FGF/ERK inhibition, GSK3ß inhibition, LIF/STAT3 activation, as well as combined treatment for 3 hours each.Overall design: EpiSCs were grown in KSR medium on low-density feeders. At semiconfluency, media were changed as detailed for the individual samples. Cells were harvested for RNA isolation 3 hours later.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: ModelOrganism
Organization: Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine
Literatures
- PMID: 20207225
Release date: 2010-02-21
Last updated: 2009-07-16