Project name: Solanum lycopersicum strain:Ailsa Craig-derived mutants

Description: We have studied tomato ripening regulation and have developed CRISPR/Cas9-mediated allelic mutations in the RIN locus, which encodes a transcription factor regulating ripening. To clarify the properties of the two allelic mutants, which were a knockout mutation (line 42) and a mutation generating an early stop codon at the amino acid position 200 while wild-type protein consisting of 242 a.a.(line G27), we performed next generation RNA sequencing (RNA-Seq) analysis of the mutant fruits that were harvested at 35 days after pollination (before ripening) and about 45 days after pollination (during ripening).

Data type: Transcriptome or Gene expression

Sample scope: Monoisolate

Relevance: Agricultural

Release date: 2020-03-11

Last updated: 2019-04-26