Description: The pharynx is an endoderm innovation in deuterostome ancestors, the descendent structure of which is a pharyngeal developmental organizer involving multi-germ layer and organ derivatives in vertebrates. However, the evolutionary origination of complicated pharynx organs in advanced vertebrates is still largely unknown. Endostyle, an evolutionarily transitional pharyngeal organ exclusively existing in basal chordates provides an opportunity to reveal the origin of pharyngeal organs. Here, by utilizing cutting-edged Stereo-seq and single-cell RNA-seq, we innovatively constructed the first single-cell resolved cell atlas in the endostyle of Styela clava, where the spatial location of Stereo-seq and high capture efficiency of single-cell RNA-seq complement each other.

Data type: Raw sequence reads; Transcriptome or Gene expression

Sample scope: Monoisolate

Release date: 2023-09-04

Last updated: 2023-04-13